def check_second(seq)
assert_equal 'chr17',seq[0].target
assert_equal 41.7, seq[0].coverage
assert_equal 100.0, seq[0].perc_identity
assert_equal 0, seq[0].mismatch
assert_equal 0, seq[0].indels
assert_equal 12, seq[0].q_start
assert_equal 26, seq[0].q_end
assert_equal -1, seq[0].strand
assert_equal 21154428, seq[0].start
assert_equal 21154442, seq[0].end
assert_equal 1, seq[0].exons
assert_equal nil, seq[0].aa_change
assert_match(/TTCGTATACCGTATT/,seq[0].aln)
assert_equal 'chr11',seq[1].target
assert_equal 52.8, seq[1].coverage
assert_equal 94.7, seq[1].perc_identity
assert_equal 1, seq[1].mismatch
assert_equal 0, seq[1].indels
assert_equal 9, seq[1].q_start
assert_equal 27, seq[1].q_end
assert_equal 1, seq[1].strand
assert_equal 99537167, seq[1].start
assert_equal 99537185, seq[1].end
assert_equal 1, seq[1].exons
assert_equal nil, seq[1].aa_change
assert_match(/AGGCATGCATGGCCCGAAC/,seq[1].aln)
assert_match(/ACGCATGCATGGCCCGAAC/,seq[1].aln)
assert_equal 'chr22',seq[2].target
assert_equal 100.0, seq[2].coverage
assert_equal 100.0, seq[2].perc_identity
assert_equal 0, seq[2].mismatch
assert_equal 0, seq[2].indels
assert_equal 1, seq[2].q_start
assert_equal 36, seq[2].q_end
assert_equal -1, seq[2].strand
assert_equal 57923909, seq[2].start
assert_equal 57926444, seq[2].end
assert_equal 2, seq[2].exons
assert_equal nil, seq[2].aa_change
assert_match(/R A P R R A G E G R G \*/,seq[2].aln)
assert_match(/CGCGCACCTCGGCGTGCAGGTG\.\.\.CAGGTGAAGGGAGAGGATGA/,seq[2].aln)
assert_match(/CGCGCACCTCGGCGTGCAG 2500 GTGAAGGGAGAGGATGA/,seq[2].aln)
assert_equal 57912718,seq[2].gene_start
assert_equal 57926714,seq[2].gene_end
assert_equal 507939,seq[2].gene_id
end