Load image to PhotoImage : PhotoImage « Tkinker « Python Tutorial

1. Python Tutorial
2. Tkinker
3. PhotoImage

from Tkinter import *
root = Tk()
frame = Frame(root)
myimage = PhotoImage(file="new.gif")
b = Button(root)
b.config(image= myimage)   # or  b.image = myimage 
frame.pack()
b.pack()
root.mainloop()